Peptide Guide
A comprehensive guide to reconstituting, storing, and handling research peptides for laboratory use.
Research Use Only. This guide is provided for educational and laboratory reference purposes. All ASCEND products are intended strictly for research use and are not for human consumption.
Understanding Lyophilized Peptides
Most research peptides are supplied in lyophilized (freeze-dried) form— a dry powder in a sealed vial. Lyophilization preserves the peptide's stability and extends shelf life. Before use in research, the peptide must be reconstituted (dissolved) in an appropriate solvent.
What You Need
Lyophilized Peptide Vial
Your ASCEND research peptide
Reconstitution Solvent
BAC water or sterile water
Insulin Syringes
For precise measurement
Alcohol Swabs
For sterilizing vial tops
Reconstitution Steps
Clean the vial top
Wipe the rubber stopper of both the peptide vial and solvent with an alcohol swab. Allow to dry.
Draw the solvent
Using a syringe, draw the desired amount of bacteriostatic water (BAC water) or sterile water. A common amount is 1-2ml, depending on the desired concentration.
Add solvent to the peptide vial
Insert the needle into the peptide vial and slowly release the water along the inside wall of the vial. Do not squirt directly onto the powder — this can damage the peptide. Let the water trickle down gently.
Allow to dissolve
Let the vial sit for a few minutes. The peptide should dissolve on its own. Do not shake — gentle swirling is acceptable if needed. Shaking can denature the peptide and reduce its effectiveness.
Store properly
Once reconstituted, store the vial in the refrigerator (2-8°C). The solution is now ready for research use.
Reconstitution Calculator
To calculate concentration after reconstitution:
Example: A 10mg (10,000mcg) peptide reconstituted with 2ml (200 units) of BAC water = 10,000 ÷ 200 = 50mcg per unit.
Storage Guidelines
Unreconstituted (Powder)
- Freezer (-20°C): up to 24 months
- Refrigerator (2-8°C): up to 6 months
- Room temperature: up to 30 days
Reconstituted (Solution)
- Refrigerator (2-8°C): up to 30 days (BAC water)
- Refrigerator (2-8°C): up to 48 hours (sterile water)
- Do not freeze reconstituted peptides
Best Practices
- Always use sterile equipment and clean technique
- Avoid repeated freeze-thaw cycles for reconstituted peptides
- Keep peptides away from direct sunlight and heat
- Use BAC water over sterile water if the peptide will be used over multiple days
- Label reconstituted vials with the date and concentration
- Do not use a peptide solution that appears cloudy or discolored
Choosing a Solvent
| Solvent | Best For | Shelf Life |
|---|---|---|
| Bacteriostatic Water (BAC) | Most peptides, multi-use vials | ~30 days refrigerated |
| Sterile Water | Single-use applications | ~48 hours refrigerated |
| Acetic Acid (0.6%) | Peptides with solubility issues (e.g., GHK-Cu) | ~30 days refrigerated |
ASCEND offers Acetic Acid in our store for peptides that require it.
Need help?
If you have questions about reconstitution or handling, our team is happy to help.
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